Dr. Ajai Prakash Gupta

Dr. Ajai Prakash Gupta

Principal Technical Officer

Quality Management & Instrumentation Division

CSIR – Indian Institute of Integrative Medicine,Canal Road, Jammu – 180001
Email: apgupta@iiim.res.in

Profile

Bio Sketch

More than 27 years of post-PhD experience in the area of Analytical Chemistry of Natural Products and Pharmaceuticals. Expertise in method development,  identification, validation  and quantification of bioactive constitutions of microbes, medicinal plants and tissue culture samples using sophisticated equipments like GCMS, UPLC, HPTLC, LC/MS/MS, LC-MS-TOF and HPLC. His area of expertise also includes analysis of Pharmo-kinetics & Pharmo-dynamics (PKPD) samples, drug metabolites and their mechanistic studies.  Published more than 100 research papers in journals of National and International repute, 10 best paper awards, 31 invited lectures,  attended 26 conferences national & International and 9 book chapters.

Attended several related courses/workshops involving awareness program for NABL assessors on ISO/IEC 17025:2005 to ISO/IEC 17025:2017, Chandigarh, NABL conclave, New Delhi, total Quality control & ISO 9000, awareness programme on ISO 9001:2000 QMS, at IHBT, Palampur (HP), Internal Auditors Course on ISO 17025:1999 QMS, at IHBT, Palampur, 12 days training at Indian Pharmacopoeia Commission, Ghaziabad, National Conference on Good Laboratory Practice (GLP), at CSIR-Industrial Toxicological Research Centre, Lucknow and workshop on Validation of Analytical methods FDA and EU, Anacon, Mumbai. He has attended International Symposium on Drug Metabolism & Pharmacokinetics (DMPK) at NIPER, Mohali, Indo-USP workshop on Pharmacopoeial Monographs of Ayurvedic/Herbal Medicines organized by PCIM & H and USP, USA at Delhi, NABL  5 days Assessor training on Laboratory Assessors Course as per ISO/IEC 17025:2005 at NITTR, Chandigarh  organized by NABL, Gurgaon.

Attended training on Q-TOF micro small molecules operator training course, at Manchester (UK), training GC-MS Techniques, Troubles-shooting and GCMS solution in GCMS-QP2010”, at Shimadzu (Asia Pacific) Pvt Ltd, Singapore, training at Indian Pharmacopoeia Commission in Analytical Division, IPC, Ghaziabad, advance workshop on Patent Strategies, at Human Resource Development Center (CSIR), Ghaziabad. Dr Gupta has also worked in Govt. Opium & Alkaloid Works, Neemuch in the analysis of opium samples and its derivatives using GC, HPLC, GC-MS and conventional methods. He has worked on projects to improve the efficiency of production plant and recovery of valuable alkaloids in process house, development of process of manufacturing value-added synthetic derivatives of morphine, codeine and thebaine viz. Oxycodone, Hydrocodone, Hydromorphone, Pholcodeine, Dihydro-codeine, Dihydro-morphine etc.

Experience has fetched him several coveted positions as technical Expert Committee member of Indian Pharmacopoeia Commission, India, Central University of Punjab (Bhatinda), Forest Research Institute, Dehradun, Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh Krishi Vishawavidhalaya, Palampur, Association of Analytical Chemist (AOAC) International, USA, Centre of Aromatic Plants, (Uttarakhand Govt.), Dehradun, Jammu & Kashmir approved Quality Control person in herbal & Pharma, Biotechnology division of Jammu University, Drug & Food Control Organization, J & K and SKAUST-Jammu.

Education

Degree Subjects University Qualifying Year
Ph D. ANALYTICAL CHEMISTRY CSIR-CIMAP-LUCKNOW 1998
M. Sc. ORGANIC CHEMISTRY B. N. COLLEGE 1989
PGDCA COMPUTER APPLICATION KNIT 1988
TOTAL QUALITY CONTROL ISO 9000 TOTAL QUALITY MANAGEMENT AND ISO 9000 ALL INDIA INSTITUTE OF MANEGEMENT STUDIES 1997
BACHLOR OF EDUCATION (B. Ed.) MATH & SCIENCE KANPUR UNIVERSITY 1996

Position Held

Position Held

Position Held Period Organization
Senior Technical Officer 10/11/2012 till date CSIR-IIIM
Senior Technical Officer B 12/26/2007~10/10/2012 CSIR-IIIM
Technical Officer B 10/11/2002~10/10/2007 CSIR-IHBT
Research Officer 09/22/2000~10/11/2002 GOVT OPIUM & ALKALOID WORKS
Senior Technical Assistant 12/04/1998~09/21/2000 GOVT OPIUM & ALKALOID WORKS

Area of Expertise

Area of Expertise

  • Analysis of raw material, tablets, capsule from commercial Pharmaceutical companies using HPLC, GC-MS, Dissolution, Disintegration, TOC, Liquid Particle counter, Karl-fisher, Particle size analyzer, assay, RS, Impurities, Residue solvents etc.
  • Method development, identification, validation, and quantification of bioactive constitutions of microbes, medicinal plants tissue culture samples using sophisticated equipments like GCMS, UPLC, HPTLC, LC/MS/MS, LC-MS-TOF and HPLC.
  • Area of expertise also includes analysis of Pharmo-kinetics & Pharmo-dynamics (PKPD) samples, drug metabolites and their mechanistic studies.

Project Involved / Ongoing Projects

Projects

  • Deciphering the mechanism(s) of host-endophytes’ coevolution, enhanced secondary metabolite production and crop productivity (MLP0048)
  • Transcriptome-wide mining of ABC gene family members and their role in stress biology of Glycyrrhiza glabra. RC approved project (MLP3012).
  • Functional genomics approach to understand  glycyrrhizin biosynthesis in Glycyrrhiza glabra (completed)

Publications and Patents

Publications

Authors Title Journal
Malik Muzafar Manzoor, Pooja Goyal, Ajai P. Gupta, Saima Khan, Priya Jaswal, Prashant Misra, Pankaj Pandotra, Ashok Ahuja, Ram A. Vishwakarma, Suphla Gupta Chemical and real-time based analysis revealed active gene machinery of glycyrrhizin biosynthesis and its accumulation in the aerial tissues of in-vitro regenerated Glycyrrhiza glabra L. Plant Growth Regulation (2020) 92:263–271
Inshad Khan, Ajai Prakash Gupta, Utapal Nandi, Bhavana vij, Sumit Roy, Samdarshi Meena, Prasoon Gupta, Anil Kumar Katare, Sundeep Jaglan, Ram Vishwakarma, Surjeet Singh, Manoj Kumar Tikko, Arvind Kumar Yadav,  Amarinder Singh, Durga Prasad Mindala, Deepika Singh, Gurdarshan singh, Preclinical development of gastro-protective botanical candidate from Woodfordia fruticosa: Chemical standardization, Efficacy, Pharmacokinetics and Safety pharmacology Journal of Ethnopharmacology, 2019.

 
Debasis Nayak, Archana Katoch, Deepak Sharma, Mir Mohd. Faheem, Souneek Chakraborty, Promod Kumar Sahu, Naveed Anjum Chikan, Hina Amin, Ajai Prakash Gupta, Sumit G. Gandhi, Debaraj Mukherjee, Anindya Goswami Indolylkojyl methane analogue IKM5 potentially inhibits invasion of breast cancer cells via attenuation of GRP78 Breast Cancer Research and Treatment. https://doi.org/10.1007/s10549-019-05301-0.

 
Rauf Ahmad Najar, Nissar Ahmad Wani, Javeed Ahmad Bhat, Nawab John Dar, Beenish Rahat, Ajai Prakash Gupta, Jaspreet Kaur, Jyotdeep Kaur, Abid Hamid Modulation of dietary folate with age confers selective hepatocellular epigenetic imprints through DNA methylation The Journal of Nutritional Biochemistry , 53, 121-132, 2017. DOI: 10.1016/j.jnutbio.2017.10.007.

 
Tareq A Wani, Shahzad A Pandith, Ajai P Gupta, Suresh Chandra, Namrata Sharma, Surrinder K Lattoo Molecular and functional characterization of two isoforms of chalcone synthase and their expression analysis in relation to flavonoid constituents in Grewia asiatica L PloS one, 12, e0179155, 2017.

 
A Magotra, A Sharma, AP Gupta, P Wazir, S Sharma, PP Singh, MK Tikoo, Ram A Vishwakarma, Gurdarshan Singh, Utpal Nandi Development and validation of a highly sensitive LC-ESI-MS/MS method for estimation of IIIM-MCD-211, a novel nitrofuranyl methyl piperazine derivative with potential activity against tuberculosis: Application to drug development Journal of Chromatography B, 1060, 200-206, 2017. https://doi.org/10.1016/j.jchromb.2017.06.015
N. Mupparapu, S. khan, S. Battini, M. Kushwaha, A. P. Gupta, N. A. Qazi, R. A. Vishwakarma Metal Free Oxidative Amidation of 2-Oxoaldehydes: A Facile Access to α-Ketoamides Organic Letters, DOI: 10.1021/ol5000204 , 2014
Aruri, Hari Prasad, Singh, Umed, Kumar, Sanjay, Kushwaha, Manoj, Gupta, Ajai Prakash, Vishwakarma, Ram, Singh, Parvinder Pal I2/Aqueous TBHP-Catalyzed Coupling of Amides with Methylarenes/Aldehydes/Alcohols: Metal-Free Synthesis of Imides Organic Letters, 2016.  18, 3638-3641. DOI: 10.1021/acs.orglett.6b01684
Shahzad Pandith, Niha Dhar, Satiander Rana, Wajid Bhat, Manoj Kushwaha, Ajai Prakash Gupta, Manzoor Shah, Ram Vishwakarma, and Surrinder Lattoo Characterization and functional promiscuity of two divergent paralogs of Type III plant polyketide synthases from Rheum emodi Wall ex. Meissn. Plant Physiology, 2016. DOI: 10.1104/pp.16.00003.
P Das, K Ranjith Reddy, A. P.  Gupta, “Metal-free aminooxygenation of alkynes: Efficient synthesis of 3-aroylimidazo [1,2-a]N-heterocycles Metal-free aminooxygenation of alkynes: Efficient synthesis of 3-aroylimidazo [1,2-a]N-heterocycles Asian Journal of Organic Chemistry, 2016. DOI: 10.1002/ajoc.201600120.

 

 

Patents:

 sno. Inventors/ Contributors Title Description Year
1. Sharma, J. R., Lal, R. K., Gupta, A. P., Mishra, H. O., Pant, V., and Mohd. R. Opiumless and alkaloid- free non-narcotic opium poppy (Papaver somniferum) variety “Sujata”. US Patent No. US6730838
2. Gupta, A. P., Singh, D. V., Gupta, M. M. and Sushil Kumar A process for extraction of stable green dye from Eclipta alba

 

 Application 234/DEL/1999 published 2005-06-03, filed 1999-02-12.
3. Sandal, I., Bhattacharya, A., Ravindranath, S. D., Singh, B., Kaul, V. K., Saini, U., Kumar, J. K., Ahuja, P. S., and  Gupta,  A. P. Use of 2-Amino glutarimide (3-amino piperidine 2,6 dione) in promoting Agrobacterium   tumefacines   mediated   genetic   transformation   of   recalcitrant   plants   and explants and a simple method of producing it File no.-0405NF2005/IN
4. Bharate Sandip Bibishan, Singh Surjeet, Singh Gurdarshan, Jain Shreyans Kumar, Kumar Ajay, Singh Bikarma,Gupta Ajai Prakash, Anand Rajneesh, Singh, Amarinder, Kushwaha Manoj, Gupta Mehak, Sharma Gourav, Sharma Ashwani & Vishwakarma Ram Standardized Extract of Bergenia ciliata for the Treatment of Inflammatory Disorders
5. Bharate Sandip Bibishan, Singh Surjeet, Singh Gurdarshan, Jain Shreyans Kumar, Kumar Ajay, Singh Bikarma,Gupta Ajai Prakash, Anand Rajneesh, Singh Amarinder, Kushwaha Manoj, Gupta Mehak, Devi Sunita, Vij Bhavna & Vishwakarma Ram Standardized Extract of Dysoxylum binectariferum for the Treatment of Inflammatory Disorders
6. Naresh Satti, Prabhu Dutt, Balkrishan chandan, Neelam Sharma, Surjeet Singh, Gurudarshan singh, Manoj Tikku, Subhash Sharma, Jyotsna suri,  Ajai Prakash Gupta, Inshad Ali Khan, Ram Vishwakarma Process for the preparation of Actioside in-rich fraction from Colebrookea oppositifolia for the treatment of alcoholic hepatitis

Awards / Honours

Awards and Honours

1.IInd best paper “P. D. Shetti award” on application of TLC/HPTLC in Pharma analysis & standardization of herbal medicines in           the field of planar chromatography; 2006. V. Pathania, A. P. Gupta, and B. Singh, “Improved HPTLC Method for Determination            of Curcuminoids from Curcuma longa”, J. of liquid chromatography and related technologies, 29, 877-885, 2006.

  1. IIIrd best paper “P. D. Shetti award” on application of TLC/HPTLC in Pharma analysis & standardization of herbal medicines in the field of planar chromatography; 2007 V. Sharma, A. P. Gupta, P. Bhandari, R. Gupta & B. Singh, “A Validated and Densitometric HPTLC Method for the Quantification of Withaferin- A & Withanolide-A in Different Plant Parts of Two Morphotypes of Withania Somnifera” Chromatographia 66 (9 – 10), 801 – 804, 2007.
  2. Best paper “P. D. Shetti award” on application of Chromatography in Pharma analysis & standardization of herbal; 2009. P. Bhandari, N. Kumar, B. Singh, A. P. Gupta, V. K. Kaul and P. S. Ahuja,“Stability-indicating LC-PDA method for determination of picrosides in Hepato- protective Indian herbal preparations of Picrorhiza kurroa ”Chromatographia, 69, 221-227, 2009.
  3. IInd best paper “P. D. Shetti award” on application of TLC/HPTLC in Pharma analysis &standardization of herbal medicines in the field of planar chromatography; 2009. N. Sharma, U. Sharma, A. P. Gupta, Devla, A. K. Sinha, B. Lal and P. S. Ahuja,“Simultaneous densitometric determination of shikonin, acetylshikonin and •- acetoxyisovaleryl-shikonin in ultrasonic-assisted extracts of four Arnebia species using reversed-phase thin layer chromatography”, J. of Separation Science, 32, 3239-3245, 2009.
  4. Appreciation award for research paper “P. D. Shetti award” on application of TLC/HPTLC in Pharma analysis & standardization of herbal medicines in the field of planar chromatography; 2009. I. Khan, P. Pandotra, A.P. Gupta, R. Sharma, B. D Gupta, J. K. Dhar, G. Ram, Y. S. Bedi, S. Gupta, “RP-Thin layer chromatographic method for the quantification of three gingerol homolog’s of ultrasonic-assisted fresh rhizome extracts of Zingiber officinale Collected from North Western Himalayas”, J. of Separation Science, 33, 558-563, 2010.
  5. IIIrd Best Paper Awards for the year 2009-10 (Indian Institute of Integrative Medicine, Jammu), I. Khan, P. Pandotra, A.P. Gupta, R. Sharma, B. D Gupta, J. K. Dhar, G. Ram, Y. S. Bedi, S. Gupta, “RP-Thin layer chromatographic method for the quantification of three gingerol homolog’s of ultrasonic-assisted fresh rhizome extracts of Zingiber officinale Collected from North Western Himalayas”, J. Separation Science, 33, 558-563, 2010.
  6. 6th Dr. P.D. Sethi’s Best paper award instituted by KONGPOSH Publications, publishers of The Pharma Review and Indian Pharma Reference Guide for the best research paper published in the field of Pharmaceutical Analysis for the year 2010. U. K. Sharma, N. Sharma, A. K. Sinha, N. Kumar, A. P Gupta, “Ultrafast UPLC-ESI-MS and HPLC with Monolithic Column for determination of Principal flavor Compounds in vanilla pods” Journal of Separation Sciences, 2009, 32, 3425-3431.
  7. Best Poster in the National Seminar on Recent advances in Plant Biotechnology: Prospects and Potentials-2011. Mohd. K. Husain, P. Pandotra, Gandhiram, S. Gupta, A. P. Gupta and Y S Bedi (2011) “Analysis of genetic diversity in North- western Himalayan ginger(Zingiber officinale) using IRAP &REMAP marker technique”, (AMU, Aligarh).
  8. III rd Best Paper Awards for the year 2012-13 (Indian Institute of Integrative Medicine, Jammu) R. Mudududdla, S. K. Jain, J. B. Bharate, A. P. Gupta, B. Singh, R. A. Vishwakarma and S. B. Bharate, “Ortho-Amidoalkylation of Phenols via Tandem One-Pot Approach Involving Oxazine Intermediate”, J. Org. Chem., 2012, 77, 8821−8827.
  9. III rd Best Paper Awards for the year 2013-14 (Indian Institute of Integrative Medicine, Jammu) N. Mupparapu, S. khan, S. Battini, M. Kushwaha, A. P. Gupta, N. A. Qazi, R. A. Vishwakarma, “Metal Free Oxidative Amidation of 2-Oxoaldehydes: A Facile Access to α-Ketoamides”, Organic Letters, DOI: 10.1021/ol5000204 , 2014.
  10. Best Oral Presentation award in Advances and Innovations in Biotechnology and Biological Sciences SBBS-2017
  11. Best Poster award in the 3rd International Conference on Recent Trends and Advancements in Engineering and Technology (ICRTAET).

13 .Best  Student Oral Presentation in International Conference Virtual Conference on Plant Specialized Metabolism and       Metabolic Engineering, 2020 at CSIR-CIMAP.

Group

Students