Quality Control & Quality Assurance

Overview

Welcome to Quality Control and Quality Assurance

QC&QA at IIIM, Jammu has developed an expertise in the specialized area of Analytical Chemistry, Food  Chemistry, Quality Evaluation of Natural/ Herbal products and finger printing of Herbal formulations.  As such QC&QA is engaged in the analysis of trace elements, heavy metals, aflatoxins and pesticide residues in honey, water, soft drinks, alcoholic and non alcoholic beverages, herbal drugs, walnut and other nuts/ products, spices and condiments and animal/poultry feed etc.

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Mission and Goals

Missions and Goals

  • To deliver basic and applied research outputs of international quality in selected branches of chemical and biological science.

  • To render analytical services of highest quality associated with degree of professional satisfaction and confidence to the customer.

  • All Laboratory personnel engaged in testing activities undertake to familiarize themselves with quality documentation and implement policies and procedures in their work.

  • Societal:
    • Testing & Monitoring of Quality of potable water in the state.
    • Assessment of industrials effluents especially effluents from pesticide  industries and its effect on ground water table, crops & population residing adjacent  to pesticide industry.
    • Services to honey industry in India, which has helped the country to check the export of honey with non-conformities which in turn could result in trade restriction.
  • Support to Scientists of IIIM in their projects by way of quality testing/quantification of marker compounds in their products and product intermediates at various stages of R&D.

Competencies

  • Research and Development activity primarily focused on development of analytical methods of analysis and testing of organic and inorganic materials
  • Identification and authentication of Medicinal & Aromatic Plants
  • Quality Control ,evaluation and Chemistry Manufacturing & Control of herbal drugs.
  • Quality Control of Essential oils
  • Quality control of Food products
  • Analysis of portable water and municipal wasters (Chemical and biological)
  • Analysis of minerals
  • Analysis of pesticides& pesticide residue
  • Analysis of microbial load / pathogens and toxins in food and water

Current Research

  • Method development /validation for analysis of herbal & herbal products, water, soft drinks, alcoholic and non alcoholic beverages, walnut and other nuts/ products for domestic/ export market and other food items like spices and condiments and animal/poultry feed etc.
  • Method development and validation for Residual analysis of Veterinary medicines, Pesticides, Metals & Aflatoxin in all products/matrices for human consumption or otherwise.

Chemical Testing

Food & Agriculture Product

Laboratory: INDIAN INSTITUTE OF INTEGRATIVE MEDICINE, JAMMU
Facility: Testing
Discipline(s):   Chemical
S.No.
Product(s)/ Material of test
Specific tests performed
1
Nuts and nut product

Aflatoxin

B1

B2

G1

G2

Free Fatty Acids

Peroxide value

Iodine value
2

Honey and honey products

Moisture

Specific gravity

Reducing sugar

Sucrose

Fructose-Glucose Ratio

Acidity

Ash

Lead

Copper

Cadmium

Mercury

Pesticides

Drugs
3
Alcoholic drinks & beverages

Ethanol content

Volatile acids

Higher alcohols as amyl alcohol

Esters as ethyl acetate

Aldehydes as acctaldehyde, acid

Total solids

Methyl alcohol

Total Acids

Sp. gravity
4
Animal feeds

pH

Copper

Moisture

Crude protein

Crude fiber

Crude fat

Salt as NaCl

Total ash

Acid  insoluble ash

Total carbohydrates

Reducing sugar

Non reducing sugar

Starch

Calcium

Total energy
5
Spices & condiments

Essential oil

Starch

Crude fibre

Total Ash

Acid insoluble ash
6
Nutraceuticals
6.1
Impurity Residual profiles

Toxic metals

Lead

Mercury

Arsenic

Cadmium

Pesticides

Aflatoxins

B1

B2

G1

G2
6.2

 
Energy Total Energy in K/Cal

Total Energy in K/Cal

Total carbohydrates

Total proteins

Total Sugars

Total Fat (Lipid)
6.3

Minerals & Micronutrients

Calcium

Sodium

Magnesium

Selenium

Iron

Zinc

Copper

Manganese
6.4
Vitamins

Vitamin A

Vitamin B1

Vitamin B6

Vitamin D

Vitamin K

Vitamin C

Vitamin B2

Vitamin B12

Vitamin E
6.5
Lipid profile

Fatty acid

Total saturated

Fatty acid total polysaturated

Cholesterol

Drug & Pharmaceuticals

Laboratory: INDIAN INSTITUTE OF INTEGRATIVE MEDICINE, JAMMU
Facility: Testing
Discipline(s):   Chemical
S.No.

Product(s)/ Material of test

Specific tests performed
1 Chemistry manufacturing Control (CMC) of herbal drugs Chemical identification by spectroscopic and or chromatographic fingerprinting Assay of active constituents or characteristic markers
Toxic metals
Mercury
Lead
Cadmium
Arsenic
Pesticides
Toxic metals
Mercury
Lead
Cadmium
Arsenic
Pesticides
Adventitious toxins (e.g. aflatoxin)
B1
G1
G2
G2
Solvents
Methylene chloride
Chloroform
Methanol
Hexane
Carbentetra chloride
Acetonitrile
1,2-dimethoxy ethane
Nitronmethane
1,4-dioxane
Methyle cyero hexane
Cyclohexane
Benzene
Pyridine
Toluene
Chlorobenzene
Ethylene glycol
N-N’-dimethyl-acetanide
Xylene
Sulfolane
Formanide
Tetraline
1-methyl-2-pyrrdidinone
2-Methoxy-ethanol
2-Ethoxy-ethanol
Residue on ignition/
water content
2.0 Herbal extracts HPLC Profile
and
HPTLC profile
2.1 Podophyllotoxins/
P. hexandrum		 Assay, HPLC,
HPTLC profile (qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
2.2 Vanillin/Vanilla parifolia Assay, HPLC,
HPTLC profile (qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
2.3 Saffron/ Crocus/ Preparation
E1% at 440 nm
1CM		 Water & Volatile matter at 103oC%
Crude fibre
Ash
Ash insoluble in HCl
Nitrogen
Colouring power
2.4 Curcumin/Haldi/ Curcuma/
Preparations		 Extractive value
Assay, HPLC,
HPTLC profile
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.5 Gallic acid / Ellagic acid in Amalki / Terminalia/
Preparations		 Extractive value
Assay, HPLC
(Gallic acid)
Assay,HPLC
(Ellagic acid)
HPTLC profile
(Gallic acid)
HPTLC profile
(Ellagic acid)
Heavy /Trace metals Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.6 Piperine / Pimpali / Maricha / Piper preparations Extractive value
Assay, HPLC,
HPTLC profile
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.7 Withanolids / Ashwangdha / withania preparations Extractive value
Assay, HPLC, Withanolide A
Assay, HPLC Withanone
Assay, HPLC Withferin A
HPTLC profile Withanolide A
HPTLC profile Withanone
HPTLC profile Withferin A
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.8 Pinitol / Gokhru/Tribulus preparations
HPTLC profile (qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
2.9 Tinosporasides & cordiosides / Columbin edysteroids/ Guddchi / Tinospora preparations Extractive value
Assay, HPLC, cordiosides
Assay, HPLC, columbin
Assay, HPLC,Edysteroids
Assay, HPLC Tinosporasides
HPTLC profile Tinosporasides
HPTLC profile cordiosides
HPTLC profile columbin
HPTLC profile Edysteroids
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
2.10 Boswellic acids/ Salai guggal /Boswellia preparations
Extractive value
Assay, HPLC, Boswellic acid
Assay, HPLC, β-Boswellic acid
Assay, HPLC, 11-keto- β- Boswellic acid
Acetyl 11-keto- β- Boswellic acid
β- Boswellic diol
Acetyl β- Boswellic acid
HPTLC profile (Qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.11 Shatvarin-4/ Shatavarin SafedMusali/ Asparagus preparations Assay, HPLC,
HPTLC profile (qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.12 Andrographolides/Andrographis/ preparations Assay, HPLC,
HPTLC profile(qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
Assay, HPLC,
2.13 Gugglusterone / Commiphora/ preparations Assay, HPLC,
HPTLC profile(qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
Assay, HPLC,
2.14 Agnuside / negundoside / Vitex / preparations Assay, HPLC,
HPTLC profile(qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.15 Stevioside./ rebaudoside / Stevia/ preparations Assay, HPLC,
HPTLC profile
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.16 Acteoside / Colebrrokia / Herbs / Preparations Assay, HPLC,
HPTLC profile(qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.17 Butrins / Butea/ Preparations Assay, HPLC,
HPTLC profile(qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.18 Picrosides / Picrorhiza / Preparations Assay, HPLC,
HPTLC profile
HPTLC profile
Heavy/Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
Assay, HPLC,
2.19 Catechin :
Green tea/
Accacia preprataiton		 Assay, HPLC,
HPTLC profile
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.20 10-deacetyl baccatin-III / Taxus / preparations Assay, HPLC,
HPTLC profile (qualitative)
Heavy/Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.21 Bacosides / Bacopa / Preparations Assay, HPLC,
HPTLC profile (qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.22 Carvone/
Flavonoid glycosides in Zeera / Kalazeera / herbals / preparations		 GC(Carvone)
HPTLC profile
Flavonoids
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.23 Plumierise / Plumieria / Preparations Assay, HPLC,
HPTLC profile
(qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
2.24 Amarogentin / Amarosewrin / Swertia / Preparations
Assay, HPLC,
HPTLC profile
(qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
Aflatoxins
B1
B2
G1
G2
Total Ash
Acid insoluble ash
3. Arka
3.1 Pudina Arka GC Profile:
l-Limonene
menthone
isomenthone
menthyl acetate
l-Menthol
Sp. Gravity
3.2 Yawani arka
(Ajwain Ark)		 GC Profile:
g-Terpinene
p-Cymene
Thymol
Sp. Gravity

Residues in Water & Food Product

Laboratory: INDIAN INSTITUTE OF INTEGRATIVE MEDICINE, JAMMU
Facility: Testing
Discipline(s):   Chemical
S.No.
Product(s)/ Material of test
Specific tests performed
a. Water
1. Water : Portable, domestic/ packaged/ aerated Metals & Other soluble residues
Dissolved solids
Residual Free Chlorine
Magnesium
Conductivity
Copper
Iron
Manganese
Zinc
Aluminium
Selenium
Cadmium
Mercury
Arsenic
Lead
Chromium
Calcium
Potassium
Molybdenum
Sodium
Nickel
Phosphate
2 Pesticide
b. FOOD PRODUCTS
2. Honey, herbals, fruits, vegetables, Tea, Processed food etc., soft drinks

Raw/processed food/etc.

 Metals & Other soluble residues
Copper
Iron
Manganese
Zinc
Aluminum
Selenium
Cadmium
Mercury
Arsenic
Lead
Chromium
Calcium
Potassium
Molybedenium
Sodium
Nickel
Phosphate
3. Pesticide
Fonofos
Mancozeb
Chlordane
2-4’-DDT
Hexachlorobenzene (HCB)
Coumaphos
Gamma HCH
Endrin
Phosalone
Permethrin
Azinophos methyl
Dichloroves
Beta HCH
Heptachlor- exo-epoxide
4-4’-DDE
Fenithrothin
Maneb
Dieldrin
Diazinon
Alpha HCH
Aldrin
Ethion
Heptachlor
Pirimiphos methyl
Endosulfan sulfate
4-4’-DDT
Piperonyl butoxide
Methidathion
Malathion
Zineb
Pentachloroaniline
4-4-DDD
Propoxur
Delta HCH
Heptachlor-endo-epoxide (tran)
Endosulfan (alpha+ beta)
Fenvalerate
Bromopropylate
Qunitozene
Parathion methyl
Alachlor
Chlorfenvinphos cis
Chlorfenvinphos trans
Cypermethrin
Chlorpyrifos methyl
Chlorpyriphos
Others
Deltamethrin
Vinclozolin
Amitraz
Chlorobenzilate
Tau-fluvalinate
Carbaryl
Carbofuran
Cyfluthrin
Cyhalothrin
4. Drugs
Residues of Drugs in Food and food products Honey, herbals, fruits, vegetables, Tea, Processed food etc., soft drinks

Food: Raw/Processed food/ etc

 Drugs
Chloramphenicol
Nitrofurans

Furazolidone [AOZ]
Furaltadone [AMOZ]
Nitrofurantion [AHD]
Nitrofurazone [SEM]
Sulfonamides

Sulfadimidine
Sulfadiazine
Sulfadimethoxine
Sulfadoxine
Sulfamerazine
Sulfanilamide
Sulfamethoxypyridazine
Sulfamethoxazole
Sulfathiazole
Trimethoprim
Streptomycin
Tetracyclines

Tetracycline
Oxytetracycline
Chlortetracycline
Doxycycline

Cosmetics & Essential Oils

Laboratory: INDIAN INSTITUTE OF INTEGRATIVE MEDICINE, JAMMU
Facility: Testing
Discipline(s):   Chemical
S.No.
Product(s)/ Material of test
Specific tests performed
1. Essential oils GC/GC-MS/ Profile with NIST/WIELY Library inputs
1.1 Lavender oil
Linalool
Linalyl acetate		 Assay, GC, Linalool
Assay, GC, Linalyl acetate
HPTLC profile (qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As
1.2 Analysis of Mentha oils /Menthol in menthe oil products Assay, GC
HPTLC profile (qualitative)
Heavy /Trace metals
Hg
Pb
Cd,
As

Major Facilities Available

The Division has following state-of-the-art analytical tools
1
 PerkinElmer 220 Triple-quad LC-MS/MS
4
 Shimadzu Nexera HPLC
2
5
3
6
 Shimadzu LC20AD XR HPLC with PDA and Fluorescence detector

Training

Principle & Application of HPLC

Training course on principle and applications of HPLC. The training programme begins with basics of chromatography and covers all the practical aspects of HPLC including sample preparation, method development and method validation. Following topics will be covered in this training.

  • Introduction to chromatography
  • Principles of liquid chromatography
  • Basics of HPLC
  • HPLC components and their functions
  • Applications of HPLC
  • Separation modes
  • Sample preparation
  • Method development
  • Sample analysis
  • Quantification
  • Method validation & recovery studies

Who can participate: Post Graduate students, Analysts & Laboratory Technicians.

Course Duration: 4 weeks

Course fee: Rs. 20,000/-

HPLTC & its Applications

Four week’s Practical course on applications of HPTLC. All practical aspects of operating HPTLC are covered under this course.

 

Objective

The course is designed to provide analysts with theoretical foundation of and practical experience with High Performance Thin-Layer Chromatography (HPTLC). The course teaches to understand application of various methods.

 

Prerequisite

Basic understanding of chromatography and/or experience with analytical laboratory work.

 

Course content

The course is subdivided into theoretical lectures, practical exercises, and discussions:

  • Parameters of HPTLC and their influence on analytical results (among others: stationary, mobile and gas phases)
  • Standardized HPTLC methodology as requirement for reproducible results
  • Derivatization procedures
  • Qualitative evaluation of chromatograms
  • Application of instruments and software experiments
  • Proper sample application
  • Use of different stationary phases
  • Effects of the chamber configuration
  • Application of HPTLC for the analysis of botanicals
  • Advantages and limitations of fingerprint analysis by HPTLC
  • Optimization of methods from literature
  • Application of digital imaging technology for documentation and evaluation of chromatograms
  • Stability tests of botanicals by HPTLC
  • Standardized methodology of HPTLC
  • Sample preparation and selection of method
  • Method optimization
  • Validation of qualitative methods

 

Who can participate: Post Graduate students, Analysts & Laboratory Technicians.

Course Duration: 4 weeks

Course fee: Rs. 20,000/-

Practical Course & Application of ICM-MS

The course provides comprehensive overview of the Agilent 7800 Series ICP-MS technique and ICP-MS ChemStation Agilent’s software.

Course Outline

  • Agilent 7800 series ICP-MS Principles and Operation
  • Fundamentals of ICP-MS and Agilent 7800 Design Features
  • ICP-MS ChemStation Software for Agilent 7800 Instrument Set up/Start up
  • Agilent 7800 Tuning
  • Set up and Tuning
  • ICP-MS Interferences
  • Octopole Reaction System (ORS)
  • ICP-MS ChemStation Acquisition Methods and Sequences
  • Laboratory Multi-Tune Method and Sequence
  • Data Analysis Methods and Principles of Quantification for FullQuant Mode
  • Laboratory Data Analysis Set up and Evaluation
  • Agilent 7500 Basic Maintenance

 

Who can participate: Post Graduate students, Analysts & Laboratory Technicians.

Course Duration: 4 weeks

Course fee: Rs. 20,000/-

Practical Training on Techniques & Applications of GC-MS

The course provides hands-on training on Shimadzu TQ 8050 NX GC-MS. Basics of gas chromatography and ion trap mass spectrometer are covered in the course along with identification of components and their quantification. The course comprises of following parts.

  • Introduction to gas chromatography
  • Introduction to mass spectrometry
  • Sample preparation
  • Sample introduction and types of sample injections
  • Analytical columns
  • GC detectors
  • Mass selective detectors
  • Ionisation techniques and analysers
  • Data interpretation
  • Quantitation and library search

 

Who can participate: Post Graduate students, Analysts & Laboratory Technicians.

Course Duration: 4 weeks

Course fee: Rs. 20,000/-

Training-Microbiological Analysis

The training shall be provided for the detection of microbial load from different samples and techniques.

A. Microbial Count

    1. Total bacterial count
    2. Coliform count/MPN count
    3. Yeast and mold count
    4. Enterobacteriaceae

B. Detection of pathogens

    1. E. Coli E. Coli
    2. Salmonella typhi
    3. Staphylococcus aureus
    4. Pseudomonas aeruginosa

C. Molecular characterization of the microbes for taxonomic designation

D. Long term preservation of microbes

Who can participate: Post Graduate and Graduate students of any Biological Stream

Course Duration: 10-15 days

Course fee: Rs. 20,000 (10,000 for A & B; 10,000 for C & D)

Training on LCMS-MS

The course offers hands-on training on Perkin Elmer QSight 220 LC-MS/MS. The course covers both practical and technical aspects of LC and triple quad mass spectrometer. Following points will be covered in the course.

  • Separation mechanisms
  • Columns, injectors, detectors and quantification
  • Principle of mass spectrometry
  • Ionisation techniques
  • Types of analysers
  • Liquid chromatography and mass spectrometer interface
  • Method development and optimisation
  • Quantitative and qualitative mass analysis
  • Experimental parameter optimisation

 

Who can participate: Post Graduate & PhD students, Analysts & Laboratory Technicians.

Prerequisite: Basic understanding of chromatography and/or experience with analytical laboratory work.

Course Duration: 5 days

Course fee: Rs. 20,000/-

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