Dr. Prashant Misra
Plant Sciences and Agrotechnology Division (PSA)
CSIR – Indian Institute of Integrative Medicine, Canal Road, Jammu – 180001
Dr. Prashant Misra carried out his Ph.D. studies as CSIR-JRF and SRF at Plant Gene Expression Lab, CSIR-National Botanical Research Institute in the area of Plant Molecular Biology, and was awarded the Ph.D. degree in Botany on the topic entitled “Metabolic Engineering of Non-leguminous Plants for Biosynthesis of Isoflavones”by Lucknow University, Lucknow. He has a basic training in the tools and techniques of plant molecular biology e.g. molecular cloning, gene expression analysis, transgenic plant development, plant tissue culture, bioinformatics etc. He has been recipients of Alexander von Humboldt postdoctoral Fellowship for carrying out research work at Technical Universtity of Munich, Munich (Germany).
He Joined CSIR-IIIM in the year 2012. His research interest is mainly centred on understanding of molecular regulation of plant secondary metabolism in medicinal and aromatic plants. Currently, the primary focus of his research activities is on the investigation of the gene regulatory networks in the glandular trichomes. His group studies diverse aspects of plant secondary metabolism such as transport of secondary metabolites and transcriptional regulation of plant secondary metabolism by using modern tools and techniques of molecular biology including next-generation sequencing. The overall aim of his research activities is to develop knowledge base for application in the area of modulation of biosynthesis of plant natural products.
Place of work: CSIR-NBRI
|B.Sc.||Zoology, Botany, Chemistry||Lucknow University
|Principal Scientist||18th December 2021 to till date||CSIR-IIIM|
|Senior Scientist||18th December 2017 to 17th December 2021||CSIR-IIIM|
|Scientist||18th December 2012 to 17th December 2017||CSIR-IIIM|
Area of Expertise
- The major areas of expertise include plant molecular biology, transgenic plants, plant secondary metabolism, plant tissue culture and transcriptomics. With this expertise, our research work is focussed on the following lines:
- Understanding molecular basis of regulation of plant secondary metabolism in medicinal and aromatic plants.
- Molecular biology of plant glandular trichomes
- Elucidation and metabolic engineering of pathways involved in the biosynthesis of plant secondary metabolites
4.Glycoengineering of protein glycosylation machinery through CRISPR Cas based genome editing
- Glycoengineering of Nicotiana tabacumusing CRISPR Cas based genome editing: Glyco-optimization for humanized therapeutic protein production in plants (GAP2167/DBT-funded)
- Chemistry & Plant Biotechnology of Phytocanabinoids from Cannabis sativa(MLP-1001/CSIR)
- Biotechnology of medicinal and aromatic plants (MLP-3012/CSIR)
- Development of functional genomics platform in Cannabis (CSIR)
Selected List of Publications
- Gani U, Nautiyal AK, Kundan M, Rout B, Pandey A, Misra P*. (2022) Two homeologous MATE transporter genes, NtMATE21 and NtMATE22, are involved in the modulation of plant growth and flavonol transport in Nicotiana tabacum. J Exp Bot. erac249. doi: 10.1093/jxb/erac249. (Corresponding author)
- Kundan M, Gani U, Fayaz M, Angmo T, Kesari R, Rahul VP, Gairola S, Misra P* (2022)Two R2R3-MYB transcription factors, CsMYB33 and CsMYB78 are involved in the regulation of anthocyanin biosynthesis in Cannabis sativa, Industrial Crops and Products,188, Part A,115546, (Corresponding author)
- Gani U, Sharma P, Tiwari H, Nautiyal AK, Kundan M, Wajid MA, Kesari R, Nargotra A, Misra P* (2021) Comprehensive genome-wide identification, characterization, and expression profiling of MATE gene family in Nicotiana tabacum. Gene, 783 org/10.1016/j.gene.2021.145554 (Corresponding author)
- Gani U, Vishwakrama RA, Misra P* (2020) Membrane transporters: the key drivers of transport of secondary metabolites in plants. Plant Cell Reports. (doi.org/10.1007/s00299-020-02599-9) (Corresponding author)
- Nautiyal AK, Gani U, Sharma P, Kundan M, Fayaz M, Lattoo SK, Misra P* (2020) Comprehensive Transcriptome Analysis Provides Insights Into Metabolic and Gene Regulatory Networks in Trichomes of Nicotiana Tabacum. Plant Mol Biol. 102(6):625-644. (Corresponding author)
- Rather GA, Sharma A, Pandith SA, Kaul V, Nandi U, Misra P *, Lattoo SK (2018) De novo transcriptome analyses reveals putative pathway genes involved In biosynthesis and regulation of camptothecin in Nothapodytes nimmoniana (Graham) Mabb. Plant Mol Biol. 96, 1–2, 197–21 (Joint corresponding author)
- Pandey A, Misra P*, Choudhary D, Yadav R, Goel R, Bhambhani S, Sanyal I, Trivedi R, Kumar Trivedi P (2015) AtMYB12 expression in tomato leads to large scale differential modulation in transcriptome and flavonoid content in leaf and fruit tissues. Scientific Reports doi: 10.1038/srep12412. (*Joint first author)
- Pandey, A†., Misra P†., Khan, M.P., Swarnkar, G., Tewari, M.C., Bhambhani, S., Trivedi, R., Chattopadhyay, N. and Trivedi, P.K. (2013) Co-expression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. Plant Biotechnology Journal 12(1):69-80. (†Joint first author)
- Misra P, Pandey A, Tewari SK, Nath P and Trivedi PK (2010) Characterization of isoflavone synthase gene of Psoralea corylifolia, a medicinal plant. Plant Cell Reports 29, 747-755.
- Misra P, Pandey A, Tewari M, Chandrashekhar K, Siddhu OP, Asif MH, Chakrabarty D, Singh PK, Nath P, Trivedi PK and Tuli R (2010) Modulation of transcriptome and metabolome by AtMYB12 transcription factor leads to insect tolerance. Plant Physiology 152, 2258-2268.
Awards and Honours
- CSIR JRF and SRF
- Alexander von Humboldt fellowship
- Reviewing for prestigious international journals such as Scientific Reports, Plos One, Plant Cell Reports, Plant Molecular Biology, Plant Journal, BMC Genomics, BMC Plant Biology etc.
- Recognized as review Editor on the Editorial Board of Bioinformatics and Computational Biology, part of the journal Frontiers in Bioengineering and Biotechnology, Frontiers in Genetics and Frontiers in Plant Science.
- Recognized as Ph.D. examiner of Banaras Hindu University, Varanasi, India, Panjab University, Chandigarh, India
|S NO.||Name||Area of Interest||Designation|
|Maridul Kundan||Identification and molecular characterization of R2R3-MYB family transcription factor genes from Cannabis sativa||Project Associate I
Ph.D. thesis submitted
|Priyanka Sharma||Molecular regulation of essential oil biosynthesis in plants and glycoengineering of protein glycosylation machinery through CRISPR Cas based genome editing||PhD Scholar
|Md. Fayaz||Molecular aspects of biosynthesis of cannabinoids||
|Mir Abdul Wajid||Molecular Biology of essential oil biosynthesis in aromatic plants||CSIR- SRF
|Tsering Angmo||Regulation of plant secondary metabolismMolecular biology of glandular trichomes||UGC- JRF
|Koushik Pal||Regulation of plant secondary metabolismGene regulation in glandular trichomes||UGC- JRF
|Dr. Kajal Katoch||Genome editing of Cannabis sativa||Project Associate- I|
|S NO.||Name||Research topic||Current position|
|Dr. Abhishek Nautiyal||Identification & characterization of transcription factor genes expressing in trichomes of Nicotiana tabacum||Scientist, Govt. Tissue Culture Lab.Department of Horticulture & Food Processing, LucknowPhD Awarded (2022)|
|Dr. Umar Gani||Identification and functional characterization of MATE transporter genes in the context of flavonoid transport in Nicotiana tabacum||Postdoctoral fellowship at University of British Columbia,CanadaPhD awarded (2022)|