Dr. Prashant Misra

Dr. Prashant Misra

Senior Scientist

Plant Sciences and Agrotechnology Division (PSA)

CSIR – Indian Institute of Integrative Medicine,Canal Road, Jammu – 180001
Email: pmisra@iiim.res.in

Profile

Bio Sketch

Dr. Prashant Misra carried out his Ph.D. studies as CSIR-JRF and SRF at Plant Gene Expression Lab, CSIR-National Botanical Research Institute in the area of Plant Molecular Biology, and was awarded the Ph.D. degree in Botany on the topic entitled “Metabolic Engineering of Non-leguminous Plants for Biosynthesis of Isoflavones”by Lucknow University, Lucknow. He has a basic training in the tools and techniques of plant molecular biology e.g. molecular cloning, gene expression analysis, transgenic plant development, plant tissue culture, bioinformatics etc.  He has been recipients of Alexander von Humboldt postdoctoral Fellowship for carrying out research work at Technical Universtity of Munich, Munich (Germany).

He Joined CSIR-IIIM in the year 2012. His research interest is mainly centred on understanding of molecular regulation of plant secondary metabolism in medicinal and aromatic plants. Currently, the primary focus of his research activities is on the investigation of the gene regulatory networks in the glandular trichomes. His group studies diverse aspects of plant secondary metabolism such as transport of secondary metabolites and transcriptional regulation of plant secondary metabolism by using modern tools and techniques of molecular biology including next-generation sequencing. The overall aim of his research activities is to develop knowledge base for application in the area of modulation of biosynthesis of plant natural products.

Education

Degree Subjects University Qualifying Year
Ph.D. Botany Lucknow University

Place of work: CSIR-NBRI

 2011
M.Sc. Botany Lucknow University

 

 2004
B.Sc. Zoology, Botany, Chemistry Lucknow University

 

 2001

Position Held

Position Held

Position Held Period Organization
Senior Scientist 18th December 2017 to till date CSIR-IIIM
Scientist 18th December 2012 to 17th December 2017 CSIR-IIIM

 

 

Area of Expertise

Area of Expertise

  • The major areas of expertise include plant molecular biology, transgenic plants, plant secondary metabolism, plant tissue culture and transcriptomics. With this expertise, our research work is focussed on the following lines:
    1. Understanding molecular basis of regulation of plant secondary metabolism in medicinal and aromatic plants.
    2. Molecular biology of plant glandular trichomes
    3. Elucidation and metabolic engineering of pathways involved in the biosynthesis of plant secondary metabolites

    4.Glycoengineering of protein glycosylation machinery through CRISPR Cas based genome editing

 

Project Involved / Ongoing Projects

Projects

  • Glycoengineering of Nicotiana tabacumusing CRISPR Cas based genome editing: Glyco-optimization for humanized therapeutic protein production in plants (GAP2167/DBT-funded)
  • Chemistry & Plant Biotechnology of Phytocanabinoids from Cannabis sativa(MLP-1001/CSIR)
  • Biotechnology of medicinal and aromatic plants (MLP-3012/CSIR)
  • Development of functional genomics platform in Cannabis (CSIR)

 

 

 

Publications and Patents

Selected List of Publications

  1. Gani U, Sharma P, Tiwari H, Nautiyal AK, Kundan M, Wajid MA, Kesari R, Nargotra A, Misra P* (2021) Comprehensive genome-wide identification, characterization, and expression profiling of MATE gene family in Nicotiana tabacum. Gene, 783 org/10.1016/j.gene.2021.145554 (Corresponding author)
  2. Manjoor MM, Goyal P, Pandotra P, Dar MS, Dar MJ, Misra P*, Gupta AP, Vishwakrama RA, Ahuja A, Dhar MK, Gupta S* (2021) Transcriptome-wide identification of squalene epoxidase genes from Glycyrrhiza glabra: expression analysis and heterologous expression of GgSQE1 suggest important role in terpenoid biosynthesis. Protoplasma doi: 10.1007/s00709-021-01616-2. (Joint corresponding author)
  3. Gani U, Vishwakrama RA, Misra P* (2020) Membrane transporters: the key drivers of transport of secondary metabolites in plants. Plant Cell Reports. (doi.org/10.1007/s00299-020-02599-9) (Corresponding author)
  4. Nautiyal AK, Gani U, Sharma P, Kundan M, Fayaz M, Lattoo SK, Misra P* (2020) Comprehensive Transcriptome Analysis Provides Insights Into Metabolic and Gene Regulatory Networks in Trichomes of Nicotiana Tabacum. Plant Mol Biol. 102(6):625-644. (Corresponding author)
  5. Sharma A, Rather GA, Misra P, Dhar MK, Lattoo SK (2019) Jasmonate responsive transcription factor WsMYC2 regulates the biosynthesis of triterpenoid withanolides and phytosterol via key pathway genes in Withania somnifera (L.) Dunal. Plant Mol Biol. 100(4-5):543-560. doi: 10.1007/s11103-019-00880-4. Epub 2019
  6. Rather GA, Sharma A, Pandith SA, Kaul V, Nandi U, Misra P *, Lattoo SK* (2018) De novo transcriptome analyses reveals putative pathway genes involved inbiosynthesis and regulation of camptothecin in Nothapodytes nimmoniana (Graham) Mabb. Plant Mol Biol. 96, 1–2, 197–21 (Joint corresponding author)
  7. Pandey A, Misra P*, Choudhary D, Yadav R, Goel R, Bhambhani S, Sanyal I, Trivedi R, Kumar Trivedi P (2015) AtMYB12 expression in tomato leads to large scale differential modulation in transcriptome and flavonoid content in leaf and fruit tissues. Scientific Reports doi: 10.1038/srep12412. (*Joint first author)
  8. Pandey, A†., Misra P†., Khan, M.P., Swarnkar, G., Tewari, M.C., Bhambhani, S., Trivedi, R., Chattopadhyay, N. and Trivedi, P.K. (2013) Co-expression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. Plant Biotechnology Journal 12(1):69-80. (†Joint first author) IF:7.110
  9. Misra P, Pandey A, Tewari SK, Nath P and Trivedi PK (2010) Characterization of isoflavone synthase gene of Psoralea corylifolia, a medicinal plant. Plant Cell Reports 29, 747-755.
  10. Misra P, Pandey A, Tewari M, Chandrashekhar K, Siddhu OP, Asif MH, Chakrabarty D, Singh PK, Nath P, Trivedi PK and Tuli R (2010) Modulation of transcriptome and metabolome by AtMYB12 transcription factor leads to insect tolerance. Plant Physiology 152, 2258-2268.
Full List of Publication

Awards / Honours

Awards and Honours

  • CSIR JRF and SRF
  • Alexander von Humboldt fellowship

Other recognitions:

  • Reviewing for prestigious international journals such as Scientific Reports, Plos One, Plant Cell Reports, Plant Molecular Biology, Plant Journal, BMC Genomics, BMC Plant Biology etc.
  • Recognized as review Editor on the Editorial Board of Bioinformatics and Computational Biology, part of the journal Frontiers in Bioengineering and Biotechnology, Frontiers in Genetics and Frontiers in Plant Science.
  • Recognized as Ph.D. examiner of Banaras Hindu University, Varanasi, India, Panjab University, Chandigarh, India

 

Group

Students

S NO. Name Area of Interest  Designation
Abhishekh Kumar Nautiyal
  • Gene regulation in glandular trichomes of tabacum
  • Regulation of plant secondary metabolism
      SRF
Umar Gani
  • Transporters of plant secondary metabolites Regulation of plant secondary metabolism
    SRF
Maridul Kundan
  • Molecular regulation of cannabinoid biosynthesis Trichome biology in medicinal plant
    SRF
Priyanka Sharma
  • Protein Glycosylation in plants Molecular regulation of essential oil biosynthesis
   Project assistant II
Mohd. Fayaz
  • Molecular regulation of cannabinoid biosynthesis Trichome biology in medicinal plant
  JRF
Mir Abdul  Wajid
  • Molecular biology of glandular trichomes Molecular regulation of essential oil biosynthesis
   JRF