Two lyophilisers provide drying facility for various aqueous extracts.

The extracts are dried at low temperature under vacuum.

a) Telstar make Lyobeta 35 lyophiliser

• 5 shelves
• Shelf temperature 70oC
• Condenser temperature 85oC
• Condenser capacity 30 Kg

b) Lyodryer ST lyophiliser

• 6 shelves
• Shelf temperature 70oC
• Condenser temperature 85oC
• Condenser capacity 30 Kg
• With facility for drying & sealing in vials of various capacities
• various capacities

Varian GCMS/MS model 4000 with MS workstation

Configuration: 

• 1 split/splitless injector & 2 PTV injectors
• FID detector
• MS/MS detector
• Direct sampling system (Chromatoprobe) for mass of pure samples
• Autosampler (Combipal) for liquid & HSS sampling
• Wiley Library
• Library mass finder for terpinoids & related constitue

• Agilent 1100 LC has been coupled with Bruker make mass spectrometer model Esquire 3000. Following are the details of the instrument
• LC:
• Binary Gradient pump with on-line degasser
• Autosampler with capacity of 100 samples
• Column oven
• PDA detector
• MS:
• Sample inlet systems: Liquid chromatographic system and syringe pump inlets
• Ionization sources: ESI and APCI constitute the API ionization sources and the Ion trap analyzer with MSn facility
• The instrument is used for the mass determination of pure molecules and LC-MS studies provide information about the number of molecules and their atomic masses present in the mixtures. The instrument is used both for qualitative as well as quantitative analysis

Sepiatec make Seperation box is an instrument for two dimensional separation of compounds. An extract can be separated into its various fractions and each fraction collected in 2ml capacity 96 well microtitre plates. In the first dimension, the extract is separated and adsorbed on various On-line SPE columns depending upon the polarity of the compounds. In the second dimension, these compounds are again separated on reverse phase columns. UV-visible detector and ELSD detector based collection is then performed. The instrument is totally automated.

Shimadzu 17A GC with Head Space Sampling System HSS-4A

• It is a dual channel GC with two FID detectors, two split/split less injectors and auto sampler for head space sampling applications. The instrument is extensively being used for separation of aromatic compounds and volatile mixtures utilizing capillary columns. Head space sampling enhances the application of GC by eliminating the extraction & sample preparation step. The instrument is fully programmable using Class GC 10 software & PC
• A dual channel gas chromatograph with two injectors & two FID detectors. The instrument utilizes Winacds software with Aimil chromatography data station for data acquisition & analysis

Analytical HPLCs of various makes are being used routinely for qualitative & quantitative analysis of natural products. Following are the details.

• State of- art HPLC machine of Thermofinnigan make consisting of quaternary P4000 pump, AS3000 autosampler and UV6000LP photo diode array detector. The machine is fully computer controlled and data is acquired using Chromquest software. The machine can be easily converted to semi-preparative HPLC by changing the pump heads and connecting Gilson fraction collector with it.
• Shimadzu make HPLC consisting of binary pumps LC-10 ATvp, CTO-10Asvp column oven, SIL –10Advp autosampler, SPD-M 10Avp PDA detector, RID-10A refractive index detector and SCL-10Avp communication module. The instrument works on Class 10vp software.
• Agilent 1100 series HPLC consisting of quaternary pump and PDA detector. Sedex 75 ELSD detector has been connected in series with PDA detector to enhance its detection capability
• Besides the analytical HPLCs mentioned above, two Waters preparative HPLCs are also being operated in the Division. One of the machines uses Empower software and the other works on Millennium software

The instrument gives the percentage of carbon, hydrogen, nitrogen, oxygen & sulphur in the sample. The instrument utilizes the technique of catalytic tube combustion of the weighed sample. The separation of the gases formed is done utilizing adsorption technique on different columns, which are finally detected by thermal conductivity detector. The weight of the sample to be analysed should be in the range of 0.2mg to 200mg. Following are the dynamic working ranges of the instrument.

• C: 0.03 – 20mg
• H: 0.03 3mg
• N: 0.03 3mg
• S: 0.03 6mg
• O: 0.03 2mg

Bruker make FT-IR spectrophotometer model Vector 22 . The instrument works on Opus software.

UV-Vis spectrophotometer :

Shimadzu make double beam UV-Visible spectrophotometers with spectral range of 190-800nm