The instrument consists of following configuration.

• Degasser: DGU-20A5R
• Pump: LC-20AD
• Autosampler: SIL-20AHT
• Column oven: CTO-10ASVp
• Detector: PDA SPD-M20A
• Fraction collector: FRC-10A

Working principle: Chromatography is a technique of separating mixture of substances into their components based on their composition and molecular structure. The process involves movement of sample between two phases namely stationary phase and mobile phase. The separation of various components takes place depending upon affinity of various molecules towards stationary or mobile phase. High performance liquid chromatography uses this technique and the mobile phase (solvent) is pumped through the stationary phase (column) at high pressure. This results in faster and better separation of various components.
HPLC can be used both for qualitative as well as quantitative analysis. The working of the instrument and collection of data is controlled through software. Different types of detectors can be attached with HPLC where photo diode array detector is the most commonly used detector.