HPTLC and its applications


 

Four week's Practical course on applications of HPTLC. All practical aspects of operating HPTLC are covered under this course.

   

Objective

  

The course is designed to provide analysts with theoretical foundation of and practical experience with High Performance Thin-Layer Chromatography (HPTLC). The course teaches to understand application of various methods.
   

Prerequisite

  

Basic understanding of chromatography and/or experience with analytical laboratory work.

   

Course content

The course is subdivided into theoretical lectures, practical exercises, and discussions:
 

    Parameters of HPTLC and their influence on analytical results  (among others: stationary, mobile and gas phases)

    Standardized HPTLC methodology as requirement for  reproducible results

Derivatization procedures

    Qualitative evaluation of chromatograms

    Application of instruments and software experiments

    Proper sample application

    Use of different stationary phases

    Effects of the chamber configuration

    Application of HPTLC for the analysis of botanicals

    Advantages and limitations of fingerprint analysis by HPTLC

    Optimization of methods from literature

    Application of digital imaging technology for documentation and  evaluation of chromatograms

    Stability tests of botanicals by HPTLC

    Standardized methodology of HPTLC

    Sample preparation and selection of method

    Method optimization

    Validation of qualitative methods

Who can participate: Post Graduate students, Analysts & Laboratory Technicians.
   
Course Duration: 4 weeks
   
Course fee: Rs. 20,000/-