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Instrumentation Division

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Overview

Instrumentation division of IIIM Jammu is actively involved in Pharmacokinetic/ADME studies of structurally and therapeutically different drugs, and their validation; development of validated HPLC, HPLC-MS and HPLC-MS/MS methodologies for the standardization of plant extracts, active fractions & sub fractions of herbal and poly herbal formulations; separation, identification and purification of the natural products with the help of Liquid Chromatography-Mass Spectrometer (LC-MS). Characterisation of novel coumpounds using NMR. Analysis of volatile compounds and essential oils using GC and GC-MS facilities.


Competencies

To develop HPLC, HPLC-MS methodologies for the ADME studies of structurally and therapeutically different drugs in different matrices such as bio-fluids such as blood, plasma, serum & urine and edible foods such as honey, milk and meat.
To develop HPLC-MS and HPLC methods for the standardization of plant extracts, fractions and sub-fractions, for drawing the chemical fingerprints of the plant extracts.
To develop method and analyse volatile compounds and essential oils along with their library search.

People:

Sr. NO Name Expertise E-mail
1. Rajneesh Anand Head ranand@iiim.ac.in
2. Kushal Bindu Operation of GC-MS kbindu@iiim.ac.in
3. M. K. Verma Analytical Chemistry, Operation of UPLC-Q-TOF-MS mkverma@iiim.ac.in
4. Vikram Bhardwaj Operation of HPLC,HRMS vikrambhardwaj@iiim.ac.in
5. Rajinder Gupta Operation of HPLC,HRMS rkgupta@iiim.ac.in
6. S K Ganjoo Operation of GC skganjoo@iiim.ac.in
7. Purshotam Kumar Operation of IR, Polarimeter, CHN analyser  
8. Niteen Narkhede Operation of HPLC, LCMS nanarkhede@iiim.ac.in
9. Naresh Joshi Operation of Lyophiliser nareshjoshi@iiim.ac.in
10. Shabir Sayed Operation of Liquid Nitrogen Plant shabirhusen@iiim.ac.in
11. Amit Kumar Operation of HPLC and maintaining repository amitkumar@iiim.ac.in
12. Sumit Kumar Operation of NMR sumitkumar@iiim.ac.in
13. Ashok Kumar Operation of Liquid Nitrogen Plant asharma.npc@iiim.ac.in
Area of research

Isolation and characterization of the natural products from the medicinal and aromatic plants used in the herbal preparations by using modern analytical techniques like High Performance Liquid Chromatography (HPLC) coupled with the use of spectroscopic techniques like 1H NMR, 13C NMR, LC-MS, IR and UV for structure elucidation. Besides the already known compounds a galaxy of unknown compounds having diversified structures have been isolated from the plants. Plants bearing edible colors have also been screened for the color bearing molecules.
 A large no. of plants exhibiting various pharmacological activities such as Antidepressant, Immunomodulatory, Bioavailability enhancing activity have been screened by using LC-MS.
The plants and the pure molecules analysed by HPLC, HPLC-MS and HPLC-MS/MS are as under:        
 
  • Hypericum perforatum

  • Echinacea purpurea.

  • Argyrolobium roseum

  • Vitex negundo

  • Colebrookeia oppositifolia

  • Withania somnifera

  • Boswellia serratta

  • Thevetia peruviana

  • Embelica officinalis

  • Zingiber officinalis

  • Dioscorea deltoida    

  • Podophyllum emodi       

  • Crocus sativus

  • Valerian officinalis.

  • Swertia chirayita

  • Tinospora cordifolia

  • Carum carvi    

  • Cuminum cyminum

  • Cesrtum diurnum    

  • Bacopa monneiri

  • Centella asiatica

  • Picrorrhiza kurroa

 

Pharmacokinetic studies of structurally and therapeutically different drugs were carried out in biological fluids in order to find out their therapeutic efficacy and bioavailability with and without a naturally occurring bioavailability enhancer. The most important of them are the Rifampicin, Pyrazinamide and isoniazid. These drugs have been studied both as such and in combination with piperine which was used as a bio-enhancer.

Facilities

LC-Q-q-QMS
LC-Q-TOF
HRMS
Analytical HPLCs
Preparative HPLC
Lyophiliser
Liquid Nitrogen Plant
NMR 400 MHz and 500MHz
GC
GC-MS/MS
Elemental Analyser
FT-IR spectrophotometer
Polarimeter

Current Research

We are engaged in development of analytical methodologies for the analysis of drugs in different matrices such as plant extracts monoherbal and polyherbal formulations, biofluids such as blood, serum and urine. Under the golden triangle project (GTP) we are standardizing number of monoherbal and polyherbal formulations. The methodologies for the HPLC and HPLC-MS analysis of natural molecules such as piperine, Gallic acids and withanolides were developed as these compounds form the major constituents of these herbal formulations.
LC-MS is also being used to characterize molecules on the basis of MS/MS studies. We are also developing the LC-MS methods for analysis of different drugs for pharmacokinetic studies. The plant extracts are being standardized on the basis of biomarker compounds. The different accessions of a plant species are being cultivated as well as collected from different regions of the country and these wild as well as cultivated collections are being studied for the amounts of the marker compounds present in these accessions. This type of studies provides information about the quality of the plant material which exist at a certain location. The components present in the endophytic fungi and fermentation broths are also being determined and quantified with the help of LC-MS and LC-MS/MS. The metabolites of the drugs are being studied in different biofluids with the help of HPLC and HPLC-MS

Projects

Project Title
Natural/ Synthetic compounds (INF0007)
Establishment of National Referral Lab (GAP0195)
Development / commercialization of biomolecules from plant sources (COR0023)
Development of natural products as Bioenhancers for oral care, Colgate Palmolive
Sponsored project (Project No. SSP-0406)
Natural additives for baby care wipes formulations Proctor and Gamble sponsored project (SSP0404)
Validation of traditional ayurvedic drugs Golden triangle project (GAP-401)